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Questions related from Sanat Bhadsavle
I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...
04 February 2021 9,529 3 View
I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...
03 February 2021 9,010 2 View
I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...
27 January 2021 687 5 View
I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.
09 December 2020 8,110 2 View
Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...
05 December 2020 4,877 2 View
Hybridization chain reaction (HCR), a form of non-enzymatic method for amplification of target nucleic acids. I need to design the two HCR primers. Is there any special software for doing that?
28 August 2020 1,137 1 View
Aptamers in most cases are themselves nucleic acids, generally used to target other bio-molecules. I wonder if we can design aptamers in such a way that they target other nucleic acids. Will...
07 June 2020 3,277 1 View
