Hello!

I performed a Dual Luciferase Assay using pGL3/pRL-TK system. The aim of the experiment is to compare the luciferase activity of a pGL3 plasmid containing the wild-type and mutated regulatory regions.

I try to take an equivalent amount of the wild-type and mutant plasmids for transfection. However, different methods for measuring plasmid concentration ( BioPhotometer, NanoDrop and qPCR) give a little bit different values of concentrations and their ratio, what can affect the final result.

So what method of measuring plasmid concentration do you using for Dual Luciferase Assay?

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