Hello!
I performed a Dual Luciferase Assay using pGL3/pRL-TK system. The aim of the experiment is to compare the luciferase activity of a pGL3 plasmid containing the wild-type and mutated regulatory regions.
I try to take an equivalent amount of the wild-type and mutant plasmids for transfection. However, different methods for measuring plasmid concentration ( BioPhotometer, NanoDrop and qPCR) give a little bit different values of concentrations and their ratio, what can affect the final result.
So what method of measuring plasmid concentration do you using for Dual Luciferase Assay?