3 Questions 1 Answers 0 Followers
Questions related from Alexandra Filatova
Dear colleagues, Please share your experience in using the psiCHECK-2 vector (Promega) for luciferase assay. I have experience with pGL3 and pRL vectors before and I always got results that the...
18 May 2020 5,493 0 View
Hi, I'm looking for some advice in analyzing my luciferase data. I study the effect of mutations in 5’-UTR on translation efficiency. For this I’ve cloned 5'UTR of the target gene (without (WT)...
03 March 2020 6,259 1 View
Hello! I performed a Dual Luciferase Assay using pGL3/pRL-TK system. The aim of the experiment is to compare the luciferase activity of a pGL3 plasmid containing the wild-type and mutated...
02 July 2019 7,813 2 View
