As far as i know you cannot grow Ganoderma in any culture media. The cultivation of Ganoderma is being done in China and perhaps in India but I am not sure if they get the spores from the cultivated Ganoderma species. Normally, Ganoderma grows on dead and decaying wood and trees particularly close to the river or in an area where plenty of water is available. It gives you up to 30 Billion spores a day for up to 6 months period if surrounded by moisture or high humidity.

Halo Pak I Nyoman :D

thanks for your answer, Pak. it makes me brighter than before. i mean to produce fruit bodies (basidiospore). is it impossible to produce a little fruit body in petri dish?

and until now, I can not purify ganoderma boninense. always contamination. . poor me haha

maybe i should learn a lot from you, Pak hehe

Hi, Mr. Syed.

thanks for your answer.

do you mean, that ganoderma which cultivated in china and india (in culture media or in wood decay?) not necessarily produce spores?

regards, gema

We at FPInnovations have developed a protocol to produce basidiospores in vitro for several basidimycetes (unfortunately not including GAnoderma) but it may work for that species as well. It is still hit and miss and I am also looking for additional input to improve it. One can produce fruit bodies in petridishes and the success is linked with how one chose appropriate temepratures a and temperature shocks, humidity and ultra violet light, and of course the "fairy dust". Here is our procedure and if anybody has additional suggestions for improvement I would be very happy to hear it. :

Prepare 1.5% water agar (15g agar in 1000 mL distilled water) and distribute 40mL in deep petri plates 25x100mm. Leave overnight. Next day prepare 2.5L of 1.5% MA (Fluka brand malt extract: 15g MA-Fluka and 20g Agar ind 1000mL distilled water). Pour 30mL on top of the set 1.5%water agar. Place a disc of sterilized filter paper (7 cm, #1 WHATCOM filters) in each plate while agar is still setting. Once the agar is set and cooled use small blocks of wood that were pre-colonised with target fungus (a stock I keep in freezer until needed). The size of these small wood chunks is few mm thick and 1 cm long and insert four of these 0.5 cm from the edge, at a 45° angle towards the center of the dish, equidistantly separated in the agar puncturing through filter paper. Wrap each individual plate with paraffin and incubate plates upside down (agar in top plate) at 20-25°C until mycelium has covered the surface of the plate. Parafilm preserves moisture and the more moisture. When mycelium covers the plate, remove parafilm and move plates in incubation chamber running at high humidity (80-90% at 15 C) under UV light that is set to 12 hours cycles. If you are lucky you will see development of fruit bodies on the plates. Some additional adjustment and tricks for different fungal species may be needed so lets all work on sharing these? Good luck. Adnan

Hi, Mr. Adnan,

thanks for your help, I'll try in the laboratory. I would be very happy if I can give advice, but my knowledge is not too far away, I was still an undergraduate student. I will discuss with my supervisor and will share with you about the results.

I will try it as soon as possible. may I ask you if I have difficulty in doing the experiment?

thank you

gema.

Dear Pak I Nyoman..

Wah, Can I, Pak? I would be very happy if I can visit your laboratory. Can I ask for your mobile numbers or BBM Pin?

thanks,

gema

Hallo Mr. Nachimutu.

what is LSF and SSF mean? i just heard it hehe

regards,

gema

Dear Nyoman

I wonder if you share a few of your tips you use in your lab especially what kind of substrates you think may encourage sporulation in badidiomycetes in petri disk and what particular regime of humidity and oxygen may be the most supportive of sporulation. Any insights will be greatly appreciated.:) . Regards , Adnan

"I Nyoman Aryantha · 4.83 · 2.97 · Bandung Institute of Technology: Hi again Gema...

Yes, you can produce fruiting body on Petri dishes with a right substrate and incubation (humidity and oxyen). You can come to my lab (PAU 6th floor - ITB) to learn how to culture Ganoderma in vitro.Cheers "

Dear Pak I Nyoman,

Pak, saya sangat ingin belajar tentang ganoderma. bolehkah saya main ke Lab bapak? kira-kira kapan waktu yang tepat untuk saya datang kesana?

thanks

gema

Dear Pak I Nyoman,

Okay, Pak.

Thanks,

gema