This is a agarose gel electrophoretogram of my PCR products using an SSR marker. This is just for checking if amplification is successful for all the DNA samples examined. This will still proceed to PAGE so no DNA ladder was included. If you can provide tips on how to prevent this kindly send me one. Thanks!
It could be primer dimers, it happens when primers are at least bit complementary, they start to replicate itself instead of target sequence. It could be prevented by decreasing concentration of primers or increasing annealing temperature.
Regards
Jan
Jan Tkadlec thank you for your insight. at first i also attributed this to high primer concentration and might be dimers but i prepared a blank sample. The blank showed no dimerization. Might be because amplification wasnt initialized due to absence of template. I tried lowering the primer concentration and see if anything changes. Thank you!
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