Jan Tkadlec

5 Questions 28 Answers 0 Followers

Questions related from Jan Tkadlec

Which categories of danger for shippment are blood samples?

I would like to send blood samples for testing into the lab in another country. Samples are from patients with suspected blood stream infection, paralel samples were already tested, and pathogens...

06 April 2016 7,002 1 View

Does anyone have RT PCR assay for detection and diferentiation of Candida species (C. albicans, C. glabrata, C. krusei)?

I´m looking for suitable RT-PCR assay to detect and distinguish Candida albicans, C. glabrata and C. krusei. Preferably assay compatible with Biorad CFX96 cycler and already used in clinical...

10 July 2015 7,846 1 View

How can I distinguish two bacterial species identical by the 16S DNA sequencing?

We cultivated unknown bacterial species from a clinical specimen. Result of the 16S sequencing were two species of Asaia - A. siamensis and A. bogorensis. Who can give me some tips how to...

01 June 2015 3,983 7 View

Does anyone have experience with erythromycin resistance gene detection?

I have a few S. aureus isolates expressing erythromycin and clindamycin resistance, but without presence of most common resistance genes (erm A,B,C and msrA gene). Has somebody experience with...

05 September 2013 563 5 View

What is the best way to isolate bacterial DNA from sputum samples (cystic fibrosis) for detection of pathogen (S. aureus)?

I want to optimize direct PCR detection of S. aureus. Does somebody have a good protocol or kit? Experience with comparison of efficiency of some methods or kits for isolating staphylococcal DNA...

31 May 2013 545 5 View