How can we asses if the population of cells in culture are neural stem cells or neural progenitor cells? Will progenitor cells also undergo neurosphere formation assay? Is there a way to identify a progenitor cell from a neural stem cell in culture?
Article Nitric Oxide Signaling and Neural Stem Cell Differentiation ...
will discuss it later on this topic.
I am trying to characterise GABA developmental switch in iPSC derived neurons using Ca2+ imaging. However, as I add GABA agonist, I observe the spontaneous activity of several neurons being...
08 September 2019 831 2 View
I routinely functionally assess the iPSC derived neurons using calcium imaging with Fluo-4/AM (490nm/510nm). However, have not been successful at using those same neuronal dishes for carrying out...
05 June 2019 3,616 2 View
I have TTX citrate ordered from Hello Bio. On using it at 1uM as my working concentration, it didn't show any effect. How do I test whether the toxin is actually working or not?
01 February 2018 8,647 6 View
How does the chemistry of calcium dyes work out? What happens during live-cell imaging if the the affinity of one dye to bind to calcium is higher than another?
01 February 2017 2,243 8 View
Will confocal be a better choice? Can you direct me to article talking about the overview of the same?
01 February 2017 3,598 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View