Yojet Sharma

6 Questions 5 Answers 0 Followers

Questions related from Yojet Sharma

Can addition of GABA agonist block transients in neurons(See description for details)?

I am trying to characterise GABA developmental switch in iPSC derived neurons using Ca2+ imaging. However, as I add GABA agonist, I observe the spontaneous activity of several neurons being...

09 September 2019 785 2 View

How does one utilise calcium imaged iPSC-derived neurons for immunocytochemistry?

I routinely functionally assess the iPSC derived neurons using calcium imaging with Fluo-4/AM (490nm/510nm). However, have not been successful at using those same neuronal dishes for carrying out...

06 June 2019 3,565 2 View

What markers can be used to distinguish neural progenitor cell from a neural stem cell?

How can we asses if the population of cells in culture are neural stem cells or neural progenitor cells? Will progenitor cells also undergo neurosphere formation assay? Is there a way to identify...

04 April 2018 5,639 1 View

How do I confirm whether Tetrodotoxin citrate is working or not?

I have TTX citrate ordered from Hello Bio. On using it at 1uM as my working concentration, it didn't show any effect. How do I test whether the toxin is actually working or not?

02 February 2018 8,616 6 View

What imaging setup can be useful for calcium imaging?

Will confocal be a better choice? Can you direct me to article talking about the overview of the same?

02 February 2017 3,570 1 View

What is the chemistry of calcium dyes?

How does the chemistry of calcium dyes work out? What happens during live-cell imaging if the the affinity of one dye to bind to calcium is higher than another?

02 February 2017 2,207 8 View