Will confocal be a better choice? Can you direct me to article talking about the overview of the same?
Better conventional epifluorescence than confocal fluorescence. Lots easier to use and cheaper. An inverted microscope (Zeiss) together Hamamatsu Photonics camera and acquisition software. Finally you need a filter wheel for two excitation lights.
I am trying to characterise GABA developmental switch in iPSC derived neurons using Ca2+ imaging. However, as I add GABA agonist, I observe the spontaneous activity of several neurons being...
08 September 2019 831 2 View
I routinely functionally assess the iPSC derived neurons using calcium imaging with Fluo-4/AM (490nm/510nm). However, have not been successful at using those same neuronal dishes for carrying out...
05 June 2019 3,616 2 View
How can we asses if the population of cells in culture are neural stem cells or neural progenitor cells? Will progenitor cells also undergo neurosphere formation assay? Is there a way to identify...
03 April 2018 5,665 1 View
I have TTX citrate ordered from Hello Bio. On using it at 1uM as my working concentration, it didn't show any effect. How do I test whether the toxin is actually working or not?
01 February 2018 8,647 6 View
How does the chemistry of calcium dyes work out? What happens during live-cell imaging if the the affinity of one dye to bind to calcium is higher than another?
01 February 2017 2,243 8 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View