Where to find information which medical assays would be good to develop?

Hi I would like to develop some medical assays for example for cancer prevention, or analysis of some markers for some disease which would be good for application in medicine in Georgia country....

04 May 2019 4,984 3 View

How to increase the yield of peptide (Solid phase peptide synthesis)?

I did synthesis of 2 peptides (1-20 amino acids) using MDHA Rink resin, I used HBTU (3.7g in 20ml (DMF)), HOBT (1.35g (29ml) NMP) to get 0.5 M. For each reaction I used 1 mL HBTU solution, 1ml...

10 November 2018 7,382 4 View

How to use plasmid for the production and isolation of tagged protein?

Hi all I am interesting if you want to produce some protein and have plasmid, the way is to grow it in bacteria and than isolate with antibody which will bind tag (example FLAG tag etc.) but what...

09 October 2018 8,557 5 View

How to open paid article and reed it for free?

How to view an article short term if journal is not open access? Is there any website which for example shows content but is not possible to copy?

07 August 2018 9,993 3 View

How to manipulate axis values in excel 2016?

I am interesting if somebody can suggest in Excel 2016, I have columns and want to write mine values instead this it chooses only automatic range for example from 1 to 40. I want type for example...

07 August 2018 7,201 0 View

How to stain bacterias with fluorescent marker?

I want to study if bacterias are associated with root, can anyone suggest for fluorescent marker which would be better to use? or which method is good (example some staining solution like acridine...

11 December 2017 2,208 5 View

How to produce commercially product and earn money with molecular biology laboratory?

Hi All There is opening laboratory of molecular biology in Georgia country. I am interesting which kind of product can produce (budget 10 000 dollars) molecular biology laboratory to start...

09 October 2017 9,602 0 View

How to identify bacteria species ?

I have some mix of bacteria and would like to know which species there are exactly. Should I send them for sequencing their DNA? or what would be cheap method?

06 July 2017 9,501 10 View

How to open journal paper online just too look content?

09 October 2016 4,196 4 View

How to track protein transport into the cells?

I am interesting can anyone suggest how to track protein transport into the cells not using GFP or any big markers? I would like to minimize the effect of marker (keep size and function of protein...

08 September 2016 6,764 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View