Where to find information which medical assays would be good to develop?

Hi I would like to develop some medical assays for example for cancer prevention, or analysis of some markers for some disease which would be good for application in medicine in Georgia country....

04 May 2019 4,984 3 View

How to increase the yield of peptide (Solid phase peptide synthesis)?

I did synthesis of 2 peptides (1-20 amino acids) using MDHA Rink resin, I used HBTU (3.7g in 20ml (DMF)), HOBT (1.35g (29ml) NMP) to get 0.5 M. For each reaction I used 1 mL HBTU solution, 1ml...

10 November 2018 7,382 4 View

How to use plasmid for the production and isolation of tagged protein?

Hi all I am interesting if you want to produce some protein and have plasmid, the way is to grow it in bacteria and than isolate with antibody which will bind tag (example FLAG tag etc.) but what...

09 October 2018 8,557 5 View

How to open paid article and reed it for free?

How to view an article short term if journal is not open access? Is there any website which for example shows content but is not possible to copy?

07 August 2018 9,993 3 View

How to manipulate axis values in excel 2016?

I am interesting if somebody can suggest in Excel 2016, I have columns and want to write mine values instead this it chooses only automatic range for example from 1 to 40. I want type for example...

07 August 2018 7,201 0 View

How to stain bacterias with fluorescent marker?

I want to study if bacterias are associated with root, can anyone suggest for fluorescent marker which would be better to use? or which method is good (example some staining solution like acridine...

11 December 2017 2,208 5 View

How to identify bacteria species ?

I have some mix of bacteria and would like to know which species there are exactly. Should I send them for sequencing their DNA? or what would be cheap method?

06 July 2017 9,501 10 View

How to open journal paper online just too look content?

09 October 2016 4,196 4 View

How to track protein transport into the cells?

I am interesting can anyone suggest how to track protein transport into the cells not using GFP or any big markers? I would like to minimize the effect of marker (keep size and function of protein...

08 September 2016 6,764 7 View

How to predict and study binding site os small molecule to protein?

Can anyone suggest which software or strategy is appropriate to find how binds small molecule to protein? for example some inhibitor that prohibits aggregation of some protein, how to predict...

08 September 2016 3,809 2 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View