i would like to know how can i attach antibody to sio2 surface in stronger way.
FIRST DETERMINE THE NET CHARGE ON YOUR PARTICLES
I USED BSA AS LINKER BETWEEN GOLD NANOPARTICLES AND ANTIBODY
I hope that this paper will be helpful.
https://www.researchgate.net/publication/265210343_Synthesis_and_surface_functionalization_of_silica_nanoparticles_for_nanomedicine
Article Synthesis and surface functionalization of silica nanopartic...
I would suggest to graft the NH2- groups onto the SiO2 first, then use preconditioned glutaraldehyde for strong covalent bonding between the grafted and antibody's NH2s.
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