I want to culture gut microbiota In vitro by culture method. Does anyone suggest me some papers where it explain about the materials required and best liquid media to culture?
I am sure you are aware that the complete consortium of bacteria composing the gut microbiota won't be able to be grown in lab. Still, you will be able to cultivate some of them depending on your research interests.
One interesting paper I have read on the subject is: Lau, J. T., F. J. Whelan, I. Herath, C. H. Lee, S. M. Collins, P. Bercik and M. G. Surette, 2016. Capturing the diversity of the human gut microbiota through culture-enriched molecular profiling. Genome Medicine. 8:72. DOI 10.1186/s13073-016-0327-7.
A commonly used medium for various in vitro systems to mimic the human gut environment is described in:
Macfarlane S, Woodmansey EJ, Macfarlane GT (2005) Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Appl Environ Microbiol 71: 7483–7492
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