What the error mean square (in a split plot design) is always higher for the main plot error as compared to the sub plot error ?

I know that in split plot design the main plot error is larger than the sub plot error. There is more error degree of freedom for sub plot as compared to main plot. But, in statistical analysis,...

14 December 2020 6,669 4 View

Which are the best methods used for isolation and purification of plant pathogenic fungi and bacteria ?

Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.

28 November 2020 3,365 3 View

Who is a principal investigator when postgraduate students conduct research, student or guide of the students?

It has been seen that guides who supervise and facilitate postgraduate students to complete the research study, claim to be the principal investigator or the author of the study. Although the...

12 October 2020 4,893 28 View

How health psychology is helpful to deal the daily life issues?

Psychological Impact of Health Disease on Human Behaviour. If anybody is interested they can join us.

10 August 2020 8,007 13 View

Does plasma therapy works for treatment of covid-19?

04 August 2020 8,186 11 View

In ansys CFD, simulation, Can we initialize two fluids kept in two adjacent compartment with two different temperatures?

Compartment wall temperature can easily initialzed with boundary conditions but if fluid inside it are at different temperatures?

03 February 2020 4,495 4 View

In Ansys CFD can we assign cell zone temperature conditions along with existing fluid/solid material conditions?

Cell zone conditions are about assigning a particular fluid or solid inside it, but it does not allow hot or cold conditions of fluid inside it. can we assign them in Ansys CFD simulation?

03 February 2020 5,199 4 View

How do we determine the position and orientation of a moving robot ??

How do we determine the position and orientation of a moving robot using IMU sensors such as MPU6050 or MPU9250 (i.e. accelerometer , gyroscope and magnetometer sensor data)??? kindly advise...

22 November 2019 7,825 5 View

Why overaging treament is required in continuous annealing line for production of dual phase steel?

Dual phase steel can be produced by directly quenching to room temperature from inter-critical temperature range. Then why overaging treatment is given in continious annealing line in steel...

09 September 2019 6,322 4 View

How to separate Adenosine and Inosine?

Hi everyone I have been trying to resolve adenosine and Inosine on HPLC column. The details are as follows: 1) Column dimension: 150mmX4.6mm, with 5 micron bead size. 2) Mobile phase: 20mM...

25 August 2019 3,420 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View