Who is a principal investigator when postgraduate students conduct research, student or guide of the students?

It has been seen that guides who supervise and facilitate postgraduate students to complete the research study, claim to be the principal investigator or the author of the study. Although the...

12 October 2020 4,893 28 View

I have a Second Order Independent Variable (3 Factors to be precise). How to use that for Mediation Analysis using PROCESS by Hayes?

I am unable to find a methodology to use Latent Variables which have multiple Factors , to be used in Mediation analysis using PROCESS macro in SPSS

26 July 2018 4,207 2 View

How to calculate COO- units and molar ration for carbodiimide chemistry?

I am confused about calculating COO- units, particularly for alginate in this case. Suppose the alginate molecular weight is 200 kDa. I understand that every repeating unit of the alginate...

08 October 2017 382 1 View

Plant material in milk

23 May 2017 4,463 7 View

Is it possible to isolate transmembrane protein ?

Hi, I have been trying to incorporate a solubilized transmembrane toxin protein into eukaryotic cell membrane. Upon incubation with vesicles the protein form pores and ruptures the vesicles as...

26 November 2016 1,242 3 View

Percentage of agarose gel ?

02 September 2016 7,477 2 View

Why do bubbles come out during the initial stages of freeze drying?

As soon is sample is inserted in the chamber at -80 C, bubbles starts forming and restricts the drying process. What could be done to avoid this.

02 May 2016 4,253 4 View

Isn't the term "Cardirespiratory Arrest" a description of being dead and NOT a "Cause of death" ?

17 February 2016 6,076 10 View

artificial intelligencence

sir u know about artificial limb

02 June 2011 7,368 1 View

How to do carbon coating on PbO ?

I want to prepare carbon coated PbO .

01 January 1970 3,738 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

Can I use aluminum or copper based substrate for DNA origami imaging using SEM?

As suggested in the title I want to image my DNA nanoflowers/origami using SEM. These nanoparticles are an organic-inorganic particle made out of highly packed DNA and also contain magnesium...

28 January 2021 3,343 3 View

How to calculate Total Phenolic Content and express it in mg GAE/kg?

26 January 2021 3,847 7 View