(Tryptic soy agar) TSA is most commonly used for this bacteria in most of the studies. However, I have observed that TSA agar gets contaminated easily when stored in the refrigerator for two weeks. So making the agar and storing it two weeks ahead of time is not a good idea in this case. Do we have any other agar for Ralstonia insidiosa that can last without any contamination when stored in the refrigerator for two weeks? Do we use selective agar for Ralstonia insidiosa in any case?
(I'm new to this field so please forgive any incorrect wordings. Thank you).
Kiran Sreeja Jayan
Grishma Prabhukhot
Try pouring agar plates in the laminar flow cabinet. The plates were likely contaminated during pouring. Contaminants grow slowly in the refrigerator and you will notice after a few days. Do you have contamination in every single plate?
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Kiran Sreeja Jayan
Grishma Prabhukhot
I have not used TSA, so cannot comment on the shelf life. Generally speaking, storing nutrient rich agar plates in the refrigerator for 1 month for general culturing of bacteria is not a big deal. I have stored and used LB agar plates after 6-8 weeks. I have had no problems. However, agar plates supplemented with antibiotics should not be stored in the refrigerator for too long. So, storage and usage of agar plates depends on the purpose. Do you supplement agar plates with antibiotics? Have you tried using TSA agar plates stored in the refrigerator for 2 weeks to culture bacteria? Any problems in recovering growth?
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