How to change the intracellular pH in human cell line?

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...

06 July 2019 3,879 1 View

Any suggestion for HADDOCK error???

I am trying to run docking experiment with YY1 protein DNA complex and a protein of my interest using HADDOCK SERVER 2.2 version. i am getting an error as "There was an inconsistency in your...

10 November 2015 3,709 1 View

Why are the cells in different planes in the Comet assay (when observed under the microscope)?

I was doing a comet assay using a Comet assay kit. When I try to visualize DNA of cells under the microscope, I realized the cells are not present in one plane so I could not focus all present in...

10 November 2014 9,879 5 View

Can anyone suggest a pH for my protein purification?

I want to purify my protein ( PI 7.6). When I used buffer with pH 8, my protein aggregates. What should the pH of the buffer for purifying my protein be?

05 June 2014 8,349 10 View

Gel shift assay (EMSA)

I am doing a EMSA with a short 20 bp oligo. I got complementary strands synthesized and annealed them. After annealing I run a gel and purified oligo from gel. But the yield after purification is...

05 June 2013 9,656 1 View

What is the suggested ratio of total cell lysate protein and purified bait protein for a pull down assay to identify new interacting partner ?

I have a nuclear protein which can interact with several proteins. I want to do pull down assay to fish a new interacting partner of my protein of interest. But i don't know how much of total...

08 September 2012 3,847 6 View

I have a protein, which could have some role in cancer progression. How can I check, whether my protein is oncogenic or not?

I have a Protein, which could have some role in Cancer progression. How can I check , whether my protein is oncogenic or not.

08 September 2012 504 10 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View