Gel shift assay (EMSA)

06 June 2013 1 10K Report

I am doing a EMSA with a short 20 bp oligo. I got complementary strands synthesized and annealed them. After annealing I run a gel and purified oligo from gel. But the yield after purification is very low. What could be possible reason? Can I use annealed oligo for EMSA experiment without purifying it after annealing? My oligos are not labeled.

Shivakumara Bheemanaik

You can use annealed oligos for EMSA without purifying. You can also run the oligos on the gel before anealing and then purify. After quantitation, you can aneal them accordingly.

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