8 Questions 5 Answers 0 Followers
Questions related from Ajit Prakash
Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...
07 July 2019 3,928 1 View
I am trying to run docking experiment with YY1 protein DNA complex and a protein of my interest using HADDOCK SERVER 2.2 version. i am getting an error as "There was an inconsistency in your...
11 November 2015 3,763 1 View
I was doing a comet assay using a Comet assay kit. When I try to visualize DNA of cells under the microscope, I realized the cells are not present in one plane so I could not focus all present in...
11 November 2014 9,964 5 View
I want to purify my protein ( PI 7.6). When I used buffer with pH 8, my protein aggregates. What should the pH of the buffer for purifying my protein be?
06 June 2014 8,393 10 View
I am doing a EMSA with a short 20 bp oligo. I got complementary strands synthesized and annealed them. After annealing I run a gel and purified oligo from gel. But the yield after purification is...
06 June 2013 9,694 1 View
I want to do pull down assay to fish novel interacting partner of my protein of interest. Can anyone suggest to me what ratio of total cell lysate protein (from mammalian cell line) and purified...
09 September 2012 2,792 0 View
I have a Protein, which could have some role in Cancer progression. How can I check , whether my protein is oncogenic or not.
09 September 2012 567 10 View
I have a nuclear protein which can interact with several proteins. I want to do pull down assay to fish a new interacting partner of my protein of interest. But i don't know how much of total...
09 September 2012 3,891 6 View
