How to change the intracellular pH in human cell line?

07 July 2019 1 4K Report

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such experiment?

Thanks.

Terrance Grant Johns

Chloroquine and ammonium chloride both reduce intracellular acidity.

Badges
Science topic

More Ajit Prakash's questions See All

Any suggestion for HADDOCK error???

I am trying to run docking experiment with YY1 protein DNA complex and a protein of my interest using HADDOCK SERVER 2.2 version. i am getting an error as "There was an inconsistency in your...

10 November 2015 3,786 1 View

Why are the cells in different planes in the Comet assay (when observed under the microscope)?

I was doing a comet assay using a Comet assay kit. When I try to visualize DNA of cells under the microscope, I realized the cells are not present in one plane so I could not focus all present in...

10 November 2014 10,002 5 View

Can anyone suggest a pH for my protein purification?

I want to purify my protein ( PI 7.6). When I used buffer with pH 8, my protein aggregates. What should the pH of the buffer for purifying my protein be?

05 June 2014 8,412 10 View

Gel shift assay (EMSA)

I am doing a EMSA with a short 20 bp oligo. I got complementary strands synthesized and annealed them. After annealing I run a gel and purified oligo from gel. But the yield after purification is...

05 June 2013 9,718 1 View

What is the suggested ratio of total cell lysate protein and purified bait protein for a pull down assay to identify new interacting partner ?

I have a nuclear protein which can interact with several proteins. I want to do pull down assay to fish a new interacting partner of my protein of interest. But i don't know how much of total...

08 September 2012 3,912 6 View

What is the suggested ratio of total cell lyaste and purified bait protein for successful pull down assay?

I want to do pull down assay to fish novel interacting partner of my protein of interest. Can anyone suggest to me what ratio of total cell lysate protein (from mammalian cell line) and purified...

08 September 2012 2,816 0 View

I have a protein, which could have some role in cancer progression. How can I check, whether my protein is oncogenic or not?

I have a Protein, which could have some role in Cancer progression. How can I check , whether my protein is oncogenic or not.

08 September 2012 591 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View