Help me please - Anybody from Brazil who works in the cultivation of animal cell lines?

Hello everyone, I am trying to bring a line cell of a mammary gland from Brazil to Chile. From the University of Sao Paulo, to be more specific. However, in order to bring them cryopreserved,...

09 October 2019 2,692 1 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

07 August 2018 6,046 5 View

I can select competent bacteria transformed with this plasmid using ampicillin?

Hi everyone, I want to order this plasmid (https://www.addgene.org/78311/) to Addgene, but when I review it, I can not find the promoter region of ampicillin resistance, so I deduce that the...

31 December 2017 1,373 8 View

No title

Hi all, I want to regenerate protoplasts from arabidopsis and I have a protocol that asks me for 2iP riboside which is already discontinued. Thanks in advance.

03 April 2017 2,800 4 View

Which is better for transfecting protoplasts of Arabidopsis thaliana, lipofectamine or polyethylene glycol?

Can you recommend some product that has already worked with some protocol? thank you very much in advance. I do not want to use electroporation.

11 December 2016 3,738 5 View

Can I insert a 7.5 kb operon into the genome of a plant by using the CRISPR-Cas9 technique trough HDR?

if so, which length approximately should each homology arm be? Thanks in advance!

09 October 2016 6,377 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View