Erwin Luis Muñoz Acuña

7 Questions 8 Answers 0 Followers

Questions related from Erwin Luis Muñoz Acuña

Help me please - Anybody from Brazil who works in the cultivation of animal cell lines?

Hello everyone, I am trying to bring a line cell of a mammary gland from Brazil to Chile. From the University of Sao Paulo, to be more specific. However, in order to bring them cryopreserved,...

10 October 2019 2,767 1 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

08 August 2018 6,268 5 View

What is the size of a fetal bovine fibroblast (not adherent)?

Hi, I would like to know what is the size (approximately) of a fetal bovine fibroblast when it is circular, that is, when it is not adherent. Thank you very much in advance!

07 July 2018 2,904 4 View

I can select competent bacteria transformed with this plasmid using ampicillin?

Hi everyone, I want to order this plasmid (https://www.addgene.org/78311/) to Addgene, but when I review it, I can not find the promoter region of ampicillin resistance, so I deduce that the...

01 January 2018 1,473 8 View

Can i use 6-(γ,γ-Dimethylallylamino)purine (2iP) instead of 6-(γ,γ-Dimethylallylamino)purine riboside (2iP Riboside) As a plant hormone?

Hi all, I want to regenerate protoplasts from arabidopsis and I have a protocol that asks me for 2iP riboside which is already discontinued. Thanks in advance.

04 April 2017 2,874 4 View

Which is better for transfecting protoplasts of Arabidopsis thaliana, lipofectamine or polyethylene glycol?

Can you recommend some product that has already worked with some protocol? thank you very much in advance. I do not want to use electroporation.

12 December 2016 3,806 5 View

Can I insert a 7.5 kb operon into the genome of a plant by using the CRISPR-Cas9 technique trough HDR?

if so, which length approximately should each homology arm be? Thanks in advance!

10 October 2016 6,477 5 View