Hi, what is the approximate size difference between mouse embryonic fibroblasts and mouse embryonic stem cells?
thanks.
Hi,
the cell size of MEFs can vary a lot. The average size is about 20 µm (often larger). In contrast, mESCs are smaller (about 8 µm). When you co-culture both cell types, you can distinguish them more clearly by cell shape. Fibroblasts show oblongness and cell processes, whereas ESCs are round shaped. Furthermore, ESCs form colonies that then appear larger than fibroblasts.
Hi,
the cell size of MEFs can vary a lot. The average size is about 20 µm (often larger). In contrast, mESCs are smaller (about 8 µm). When you co-culture both cell types, you can distinguish them more clearly by cell shape. Fibroblasts show oblongness and cell processes, whereas ESCs are round shaped. Furthermore, ESCs form colonies that then appear larger than fibroblasts.
Hi Anna, Thanks a lot for your reply. I have been culturing MEFs and mESCs together, but couldn't find the exact size difference. May I also ask you if you do any flow cytometry with mESCs? I'm looking for the earliest differentiation marker for mESCs- in case you have any idea..?
Thanks again.
Hello
i agree with Anna , for differentiation marker for mESCs see this link
http://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-9-5
Good Luck
Hi Pooja,
sorry, I just work with hiPSC and don't have any experience with FACS of mESC...good look anyway!
Hello Pooja,
Depending on that you mean by "earliest differentiation marker". It depends on your differentiation protocol. I have some of them to check on reverse transcription. Tell me if you need it.
Best
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