Pooja Khurana

8 Questions 22 Answers 0 Followers

Questions related from Pooja Khurana

What is the size difference between MEFs and mESCs?

Hi, what is the approximate size difference between mouse embryonic fibroblasts and mouse embryonic stem cells? thanks.

07 July 2016 8,313 6 View

Any advice for tissue culture contaminant detection kit?

Hi, I am looking to buy a kit for earliest detection of bacteria and fungus in cell culture (mESCs/ MEFs). I know these contaminants are easily detected by eye/ under the microscope. But I've been...

12 December 2015 6,806 8 View

Can anyone please suggest literature which suggest why ESCs even from the same strain of mice, may develop at different rates?

Hello there, many of us working on stem cells already know that different lines, even from same strains or from same mother develop differently at times. It would be great if I could get some...

10 October 2015 5,751 5 View

Hello. Has anyone used ‪WorldCourier‬ as the courier services for transporting any of the lab samples? If so, could you please share your experiences?

Hello, I'm planning to send some very important samples (snap frozen cell lines) to a company in the States from Southampton, UK. The company suggested to use World Courier services. But as we...

08 August 2015 4,814 10 View

What is the relation between maternal age and ESC derivation?

Hello, I was wondering if there is any literature out there, or personal experience on the derivation efficiency of mESC lines from aged (7-8 months) oocytes/ blastocysts.. I am seeing a stark...

07 July 2015 7,205 0 View

How could mycoplasma contamination interfere with developmental pathways in cell lines?

Hi, I might have potential mycoplasma contamination in my mESC lines derived so far (quite a few). The idea is (was) to study epigenetic changes in the periconception period using these cell...

11 November 2014 740 11 View

Has anyone worked with the R&D Systems Mycoprobe Kit for Mycoplasma detection?

Hi has anyone had any issues using this kit? My negative control of the kit gives a higher value than the values suggested for positive control. Both controls from the kit itself. Tested for the...

11 November 2014 9,241 1 View

How do you dissect a mouse Epiblast at 5.5dpc?

I've been trying to dissect mouse epiblast at 5.5 dpc, and am unable to even see and differentiate the embryo from the decidua. I repeatedly end up destroying the decidua. The biggest problem is,...

07 July 2014 3,584 7 View