What is the size difference between MEFs and mESCs?

Hi, what is the approximate size difference between mouse embryonic fibroblasts and mouse embryonic stem cells? thanks.

06 July 2016 8,258 6 View

Any advice for tissue culture contaminant detection kit?

Hi, I am looking to buy a kit for earliest detection of bacteria and fungus in cell culture (mESCs/ MEFs). I know these contaminants are easily detected by eye/ under the microscope. But I've been...

11 December 2015 6,721 8 View

Can anyone please suggest literature which suggest why ESCs even from the same strain of mice, may develop at different rates?

Hello there, many of us working on stem cells already know that different lines, even from same strains or from same mother develop differently at times. It would be great if I could get some...

09 October 2015 5,696 5 View

No title

Hello, I'm planning to send some very important samples (snap frozen cell lines) to a company in the States from Southampton, UK. The company suggested to use World Courier services. But as we...

07 August 2015 4,762 10 View

What is the relation between maternal age and ESC derivation?

Hello, I was wondering if there is any literature out there, or personal experience on the derivation efficiency of mESC lines from aged (7-8 months) oocytes/ blastocysts.. I am seeing a stark...

06 July 2015 7,158 0 View

How could mycoplasma contamination interfere with developmental pathways in cell lines?

10 November 2014 693 11 View

How do you dissect a mouse Epiblast at 5.5dpc?

I've been trying to dissect mouse epiblast at 5.5 dpc, and am unable to even see and differentiate the embryo from the decidua. I repeatedly end up destroying the decidua. The biggest problem is,...

06 July 2014 3,504 7 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

No title

02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View