Hello there, many of us working on stem cells already know that different lines, even from same strains or from same mother develop differently at times. It would be great if I could get some published literature on why this happens and in support of this view/ fact.
Thanks a lot.
Cheers!
Hi Sun,
I haven't yet explored the differentiation aspect of the lines in context yet, although that would also be influenced if there are differences in pluripotency state, of course. So for now, I am more or less talking about the generic pluripotency properties of these cells, viz., gene expression, proliferation rates, cell cycle analysis.. etc.
What I want to point out and also want to know more about is that while we compare 2 diff. groups in a study (e.g. maternal age- young and old), you would have differences in the properties of these ESC lines, even if they belong to the same group (e.g young or old), and not just that, but different even if they are from the same mother- even while they are treated in identical manner in vitro.
Do you understand my query now? I would also appreciate if you could suggest some literature I could look into.
Many thanks.
This is not too complicated to explain and an example would be to look at siblings from the same parents. Embryonic stem are derived typically from the blastocyst stage of the embryo. In the same way that brothers / sisters are not identical, this would make sense that we see differences between ESC lines from each unique blastocyst.
Even taking this point further, the ESC line from the same blastocyst can even be different if cultured in different labs due to the differing conditions in culture (media, passaging method, basement membrane, operator) can have a big impact on proliferating rate and differentiation potential of the lines.
Of course. I was wanting to find literature which supports and states/ shows that, specifically- on a more scientifically proven way. for mESCs.
Hello
I agree with Sun Yung .
find attachment & link may help you
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3980216/
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