In antioxidant activity , what is the role of phosphate buffer and salt in hydrogen peroxide scavenging activity?
Dear Naïrouz Benzeggouta, as a rule in biochemical tests emulate the physiological conditions in which the enzymes operate. The phosphate buffer allows to maintain an almost neutral pH (around 7.3) and the salts maintain the ionic strength in which the enzymatic activity is usually optimal.
Dear Naïrouz , you can tell about scavenger activity only in normal physiological conditions. normally cells are never growth in phosphate buffer, so conditions is not fit with optimal for tissue/cells. Which slat do you mean?
If you mean optimal nutrients as salt, the function is to keep optimal cells metabolism, because ion is a component of numerous enzymatic reaction, including ROS scavenger.
My best wishes!
Dear Naïrouz,
To maintain pH optimal [nearly optimal] for the activities of enzymes involved in scavenging of hydrogen peroxide. These conditions could also be ideal for non-enzymatic scavenging of hydrogen peroxide.
Good Luck
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