About water quality ?

What are the challenges and obstacles that we may face in modeling water quality in urban catchment ?

09 December 2020 9,437 20 View

About Observed Discharge Data?

Hi everyone, In case we do not have Observed Discharge Data, how do we judge the validity of the simulation results, or in other words, how do we judge the reliability of the model ? Thanks in...

12 November 2020 4,938 4 View

The Illumina NovaSeq 6000 - machine requirement for storage and analyze ?

The Illumina NovaSeq 6000 is a sequencer which is able to produce a big amount of data (2TB/run) . I need to know the technical setting of the machines that are used for the storage and analyze...

16 February 2018 5,061 2 View

Water quality and mosquitoes?

Hello To have solutions of water with variable rate of PH, what is the solution (acid) to be metered, provided that this acid is not toxic by what one is putting insects in the water used. cordially

03 October 2017 5,420 8 View

XRD shift peaks

Dear all, I have found that among reasons for the shifting of XRD peaks to higher two theta positions with increased annealing temperature in the nano regime are many including cation...

15 June 2017 9,137 8 View

Need of a paper?

Please, can anyone help me to find this article ? MAKI M., 1915.- On Macrohomotoma gladiatum Kuwayama.-Insect World, 19: 267-271.

01 March 2017 3,345 3 View

Do you want to be part of a Morocco-Turkey joint research cooperation program?

I am looking for Turkish academic researchers from a well known Turkish University for a joint research cooperation in energy efficiency in buildings or HD desalination using solar energy....

21 May 2014 5,678 2 View

Does anyone know why point mutations detected in some MLPA kits are not reproducible?

We have experience with glioma kits (mutations IDH and BRAF)?

17 February 2014 9,036 2 View

Is there a difference between master/worker models and master/slave models?

In distributed computing, are master/slave model any different from master/worker models, or they are the same?

21 November 2013 6,039 9 View

About the latest developments in the science of water?

Dear my Professors and Collegues, As a student professor and researcher, what is the lastest topics in water engineering recently that you think is the most interesting ? Best wishes.

01 January 1970 7,187 10 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View