the volume in the equation refer to the volume of extract needed for the assay (0.1ml) or is it the volume total of my extract (5ml)?

also, the concentration of my extract is 50mg/ml so I make a dilution of 10 times to have the final extract 5mg/ml and this is the extract that I used for the TPC assay so my dilution factor is 10?

weight of dry extract= 0.9g

GAE established from the calibration curve is 0.236

can you please help me?

Best Regards

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