What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

For acid treated montmorillonite clays what will be the result of surface area?

The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...

10 February 2021 1,581 3 View

Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

How to calculate thermal stress in cylinder if water is flowing inside the cylinder?

How to calculate thermal stress in stainless steel cylinder if water is flowing inside the cylinder?

14 October 2020 905 2 View

Can I add my research work in my profile "Solar Energy Assisted Synthesis Of Zinc Oxide Nanoflowers"?

Want to add my research work in my profile "Solar Energy Assisted Synthesis Of Zinc Oxide Nanoflowers"?

12 October 2020 9,511 2 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

In most of perovskite solar cell SCAPS simulation, Why does the value of donar density used to keep zero?

Perovskite is an Intrinsic semiconductor which comprised equal no.of hole and electrons concestration under thermal equilibrium condition. However in most of the perovskite solar cell SCAPS...

30 September 2020 1,023 5 View

Could anybody tell me what is the electronic affinity and charge carrier mobility of the ZnSSe semiconductor?

ZnS+ZnSe = ZnSSe alloy, or Even methods of how to calculate these parameters are welcomed.

23 September 2020 4,990 3 View

For assessment of the bird diversity, is there any rules such as the survey should be carried out for this number of days per month or not?

I carried out bird survey twice a month ( at interval of 15 days) for 8 months period following same transect line and point count stations. Is there any hard and fast rule or standard procedure...

13 September 2020 348 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View