What could be the problem on FOA selection?

I am making point mutation in yeast cells using two-step transplacement method: integration of a Ura+ plasmid containing the gene mutation and then pop-out one copy by FOA selection. The...

30 September 2020 7,000 3 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

17 July 2019 5,198 3 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

08 May 2019 7,051 1 View

How to perform data analysis for ranking of familiarity of foods?

the most familiar food was assigned to 1 and continue rank among the list of the foods until the least familiar food. How to perform weighted ranking or any suitable method can be suggested for...

21 September 2018 6,557 3 View

Could ssDNA template be the reason of low yield in RNA synthesis?

19 May 2018 6,896 3 View

What if the absorbance value of plant extract is higher than the range of calibration curve?

I am currently doing research on total flavonoid content. I use Quercetin as standard for my calibration curve. The regression coefficient is 0.99. I use different solvent extracts as my samples....

30 January 2018 9,342 9 View

What is the optimum temperature for flavanoid plant extraction using sonication technique?

I am currently optimizing the temperature for my plant extraction. My interest is to extract optimum flavanoid using sonication technique. Currently I am setting the temperature at room...

12 November 2017 4,035 7 View

How can I prepare 1 mL of 12N concentrated hydrochloric acid for acid hydrolysis?

The info I obtained from one journal article that I read for acid hydrolysis. Unfortunately the author does not include the molarity used for concentrated hydrochloric acid. The only info I got...

29 October 2017 7,320 3 View

How much chromosome in 1 ng / ul DNA extract ?Can this calculation?

How much chromosome in the 1 ng / ul DNA extract(Human somatic cells) ? Can this calculation?

14 May 2017 7,290 3 View

Is HEPES concentration affect the cell line growth?

Hi, I am going to culture Raji B cells, I mistakenly ordered RMPI with 25mM of HEPES but recommended media by ATCC only suggested 10mM. is this will affect the cell growth? thank you.

27 November 2016 4,662 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View