Fuyou Che

4 Questions 4 Answers 0 Followers

Questions related from Fuyou Che

How to verify the specificity of a primer pair?

I recently designed a pair of primers capable of amplifying 12 Chlamydia species with a target fragment length of 184bp, which was confirmed to be Chlamydia-specific by NCBI primer BLAST. I used...

17 March 2023 9,041 5 View

How can I purify monoclonal antibody from cell culture supernatant?

I have obtained monoclonal cells of hybridoma by cell fusion assay, it grows well in hybridoma medium which is GIBCO brand and supplemented with 20% fetal bovine serum, L-glutamine, how can I...

28 February 2023 2,177 3 View

Is the melting curve generated by fluorescent loop-mediated isothermal amplification informative?

I recently tested some samples using the fluorescent ring-mediated isothermal amplification method, but the melting temperature of the sample was about 2°C lower than the positive control. Is this...

19 February 2023 7,914 0 View

What is the reason why the control wells without the encapsulated antigen are positive in the indirect Elisa assay?

When doing the indirect ELSIA experiment, I only added the antigen dilution to the wells without adding the corresponding antigen, but the results showed a high OD, the primary antibody was the...

27 September 2022 3,700 2 View