Hello everyone,
I'm trying to measure the porosity of keratin/alginate hydrogel. I found high value (around 105%-110%) when I try to measure it using 95% ethanol. I have ever tried to use water to measure it, but interestingly, my porous material would dissolve in water, thus I changed to use ethanol. I have read many papers that many of them used 95% or 99% ethanol to measure porosity, and I am wondering if there would be any different to use 75% ethanol instead of 95% or 99% ?? I am wondering why most of them used 95%or 99%. ( I have not seen any paper using 75%, and i really want to know why???)
Thank you in advance for your help.
Dear friend Che-Wei Liu
According to a post on ResearchGate, the porosity of the hydrogel is usually determined by knowing the dry weight of hydrogel, swollen weight of hydrogel in absolute ethanol, density of absolute ethanol and volume of swollen hydrogel (https://blog.gotopac.com/2020/10/12/ethanol-vs-isopropyl-percentage-alcohol-content-for-sanitizer/.).
I found a paper that compared the porosity rate measurement methods of ethanol and demineralized water. The paper stated that for a sample with a low porosity rate (˜ 0.5%), a variation of ± 0,5 °C on the measured temperature leads to a difference of ± 9,2% on porosity rate with ethanol, whereas the same measurement using demineralized water would lead to a difference of ± 3,5% (https://biology.stackexchange.com/questions/39931/why-is-70-ethanol-preferred-for-aseptic-techniques.).
I also found that the ideal ethanol concentration for hand sanitizer by volume should be between 75 and 85%, with a target of 80% according to WHO (Article Optimization and comparison of porosity rate measurement met...
).In addition, my article may be interest to you.
Chapter Pretreatment of Cellulosic Biomass for Better Yield of Bioet...
I hope this helps!
Source:
(1) Question about The porosity mesure using the ethanol method - ResearchGate. https://www.researchgate.net/post/Question-about-The-porosity-mesure-using-the-ethanol-method.
(2) Optimization and comparison of porosity rate measurement methods of .... https://www.sciencedirect.com/science/article/pii/S2214860418307723.
(3) Ethanol vs Isopropyl Alcohol: Alcohol Percentage and Efficacy. https://blog.gotopac.com/2020/10/12/ethanol-vs-isopropyl-percentage-alcohol-content-for-sanitizer/.
(4) Why is 70% ethanol preferred for aseptic techniques?. https://biology.stackexchange.com/questions/39931/why-is-70-ethanol-preferred-for-aseptic-techniques.
(5) Porosity - Wikipedia. https://en.wikipedia.org/wiki/Porosity.
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