What could be the problem on FOA selection?

I am making point mutation in yeast cells using two-step transplacement method: integration of a Ura+ plasmid containing the gene mutation and then pop-out one copy by FOA selection. The...

30 September 2020 7,000 3 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

17 July 2019 5,198 3 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

08 May 2019 7,051 1 View

How to perform data analysis for ranking of familiarity of foods?

the most familiar food was assigned to 1 and continue rank among the list of the foods until the least familiar food. How to perform weighted ranking or any suitable method can be suggested for...

21 September 2018 6,557 3 View

Could ssDNA template be the reason of low yield in RNA synthesis?

19 May 2018 6,896 3 View

What if the absorbance value of plant extract is higher than the range of calibration curve?

I am currently doing research on total flavonoid content. I use Quercetin as standard for my calibration curve. The regression coefficient is 0.99. I use different solvent extracts as my samples....

30 January 2018 9,342 9 View

What is the optimum temperature for flavanoid plant extraction using sonication technique?

I am currently optimizing the temperature for my plant extraction. My interest is to extract optimum flavanoid using sonication technique. Currently I am setting the temperature at room...

12 November 2017 4,035 7 View

How can I prepare 1 mL of 12N concentrated hydrochloric acid for acid hydrolysis?

The info I obtained from one journal article that I read for acid hydrolysis. Unfortunately the author does not include the molarity used for concentrated hydrochloric acid. The only info I got...

29 October 2017 7,320 3 View

How much chromosome in 1 ng / ul DNA extract ?Can this calculation?

How much chromosome in the 1 ng / ul DNA extract(Human somatic cells) ? Can this calculation?

14 May 2017 7,290 3 View

Is HEPES concentration affect the cell line growth?

Hi, I am going to culture Raji B cells, I mistakenly ordered RMPI with 25mM of HEPES but recommended media by ATCC only suggested 10mM. is this will affect the cell growth? thank you.

27 November 2016 4,662 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

What is the Melting point criterion in a MD simulation?

I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.

26 January 2021 9,096 5 View

How to know homogeneity of two thermoplastic polymer composite?

I have melt mixed to thermoplastic polymers. But I want to know the homogeneity of the composite. What are the methods to determine whether the composite is homogeneous or not? Please help.

20 January 2021 9,085 5 View

How to solve melting and solidification of PCM using ANSYS Fluent?

Dear colleagues; I modeled 3D melting and solidification of phase change material filled heat exchanger and generated a (as in the picture below). First, I enabled melting and solidification...

19 January 2021 1,126 4 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

How should I create (which bands) a water mask for the NDSI index based on Sentinel-2 images? and Which Modis product should I use to validate?

Hi Everyone! I do my thesis about snow melting in Austrian Alps and I have some issues. I would like to create a water mask to filter out the water bodies from the study using Sentinel 2 images....

15 January 2021 4,560 1 View