My strain is Trichoderma atroviridae, while growing in synthetic medium it changes the culture media color from white to green and in such I did observe the metabolite production. What is the reason behind this?
Production of pigments by filamentous fungi is gaining interest owing to their use as food colorants, in cosmetics and textiles. Fungal pigment production can be influenced by medium composition. Complex media such as potato dextrose and malt extract increase pigment production. Filamentous fungi secrete diverse classes of pigments as secondary metabolites , including carotenoids, melanins, flavins, phenazines quinones. The change in colour from white to green is probably due to components in your synthetic medium. .
Production and chemical characterization of pigments in filamentous fungi
September 2015Microbiology 162(1)
DOI: 10.1099/mic.0.000168
Hi...your strain of Trichoderma atroviridae produces the greenish pigment that might be a polyketide compound. You may try to grow the strain in broth media and collect, quantify, and characterize the pigment(s).
Dear Aadarsh Madhu,
Trichoderma spp. are produce the pigment, non-indolic melanin-like polyphenol which is responsible for the colony and conidial spores turns to green that pigment may adsorbed by agar. here this article best describe the situation.Article Some chemical and structural features of the conidial wall o...
Dear Aadarsh.
Diffusable pigments can be characterstic for some Trichoderma species,although the colour of such pigments does not vary great deal in Trichoderma. Strains referable to section Longibrachiatum typically have conspicuous bright greenish-yellow pigments, at least when first isolated. Dull yellowish pigments are common in many species, but are not very distinctive. Some species are best characterized by a complete lack of pigment in reverse, whereas reddish pigments occur in reverse in a few isolates.Characteristic crystal produced in the media have been reported only for T.aureoviride.
Christian P.Kubicek and Gary E.Harman
Hi Aadarsh
You can check this reference
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=2ahUKEwjbuvrcoqnfAhXBxIsKHTA2Bq0QFjAEegQIBBAB&url=http%3A%2F%2Fmic.microbiologyresearch.org%2Fcontent%2Fjournal%2Fmicro%2F10.1099%2Fmic.0.000168%3Fcrawler%3Dtrue%26mimetype%3Dapplication%2Fpdf&usg=AOvVaw3SZz2odoPJth-a-RRA-REA
Green colour is due to production of conidia. The appearance of yellow to light orange colour may indicate production of secondary metabolites. Both production of conidia and secondary metabolites is influenced by substrate/ constituents of the medium
Thanks to all for the help! I have observed that green colour in my media is due the intensive conidiation. My media consist of ammonium sulphate, after 24 hrs it cause acidic shift ( pH decreases from 6 to 4) inside the media, as a consequence the fungal cells switched to reproductive phase. This is the reason what I observed in my case. Using 200 mM MES buffer or changing the nitrogen source to sodium nitrate terminated the problem.
Thanks you Subbaraman Sriram Laith Al-Ani Mohammed Fayyadh Thavamani Rajamani Tolulope Ewekeye Anirudha Chattopadhyay Hayat Essalmani Sylvester Peter Antai Sylvester Peter Antai Franci Aco Celar you have provided the direction to overcome this problem.
secretion of metabolites by the fungi (Producing enzymes for the media components)
Aadarsh Madhu I need more conidia production for my essays, could you tell me what medium do you use?
Good day to you all.
Thank you all for your valuable information.
Requesting to provide suitable article as I am curious to understand the concept behind it.
Thankyou.
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