I need to perform scratch assay to study migration of cancer cells upon various treatments of my molecule of interest. However, to block proliferation using mitomycin C, the dosage would vary for each cell line that i will be using. I need to understand what assay should be used to standardize this MMC dosage, whether a proliferation marker should be evaluated by Western blot or assays like MTT should be used. How to standardize the MMC concentration? Any suggestions..
Nandita Singhal
Assays like MTT will be a good choice. To standardise plot the % inhibition vs the conc. graph, where %inhibition= Average of Test ÷ Average of Control × 100. Control is when the concentration of drug is 0.
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