Hi Vivek,

I don't have any experience with it, But look at the attached document, This may help you.   or you could just stop my desk!!

Thank you very much Mitesh,

I read that document but there is not a precise protocols and recepie for the buffer to use in that protocol.

 Dr. SAKTHIDHARAN, Thanks for your answer but I guess there was some technical error and your answer was not posted. can you please re post it. 

Thanks for your help.

Intracellular Cross-linking Using Dimethyl Adipimidate

Dimethyl adipimidate (150 􏰆l, DMA; Pierce) at 15 􏰆ug/ml in 0.2 M triethanolamine, pH 8.5, was added to 75 u􏰆l of cell extract and incubated overnight at RT. The reaction was terminated by addition of 75u 􏰆l of 0.2 M glycine. Samples were analyzed by SDS-PAGE and immunoblotting.

I have used this chemical in  yeast and to crosslink Rpd3 and Hda1 (HDACs) to the complex proteins. I did as following

1-   to cross-link protein to protein, first cells were washed once with cold 1XPBS then re-suspended in a fresh solution of 10mM DMA (dimethyl adipimidate) in ice-cold 1XPBS containing 0.25% dimethyl sulfoxide (DMSO) and put in nutator at room temperature for 45 minutes. DMSO was used to make the cells more porous.

2- Then washed again with 1XPBS and re-suspended in 100ml of medium & 3 ml of Formaldehyde is added to get a final formaldehyde concentration 1%. Cell samples were left to crosslink for 10 to 16 hours on a rotating platform at a 180rpm room temperature for 45 minutes depending on your target (for Rpd3 at least 10 to 11 hours needed for second cross-linker to get maximal cross-linking while for Rad7 which make a complex with Rad16 and Abf1 45 minutes needed for 2nd cross-linker  Formaldehyde). but for DMA only 45 minutes needed.

you can get a detailed protocol from  Kurdistani et al (In vivo protein–protein and protein–DNA crosslinking for genomewide binding microarray). they are a pioneer in doing that. I attached the file

hope you get what you looking for

Hamed