My colleagues, I want to remove embryo from mice in many days of pregnancy period,now I have some question:
1.Is there a method for exact detection of days in pregnancy period except of seeing vaginally plaque?
2. After removing embryo, which solutions I must add for dehydration and fixation?
Thanks.
Hi. Atefeh
first the most accurate and suitable methods to confirm mice pregnancy is the vaginal plug and if you will not use early embryonic stages from 5-7 days you can confirm from abdomen size
2- the methods of fixation depends mainly on what kind of experiments you are going to do? please, let me know what kind of experiment so that i can help you
Thanks
Hi Amr Refaat
I want to prepare sections from embryo completely for Immunohistochemistry ,not only one tissue.
Another question is, in which day we can see embryos in uterus with eye.Because I want them in early embryonic days.
thanks for your replying.
1-Vaginal swabs were performed to detect the presence of male stomata in the vaginal swab, where their presence in the swab was a sign of pregnancy (Eveline et al., 2002).
2-The embryo sample is initially kept after being removed from the animal in 10% formaline solution (formalin 38% 100 ml in 900 ml tap water)
After 48 hours, it is extracted from formalin and washed several times with water.
(Picric acid 75 ml, formalin 25 ml and 5 ml acetic acid) for 12-24 hours depending on sample size and then washed with 70% ethyl alcohol to remove the yellow color followed by a series of processes depending on the method described in Suvarna et al. al., 2013).