Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View

Why do mammalian cells in culture undergo necrosis?

I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...

05 November 2020 9,280 3 View

How to purify polymer-peptide complex?

I am trying to conjugate a 12 amino acid peptide (MW 1565Da) to PLGA-PEG-maleimide copolymer (MW: 35097Da). The conjugation reaction takes place in ACN: DMF as a solvent. Can someone please...

19 October 2020 636 5 View

Can we simulate blockchain network in NS#?

I am new to ns3 and I want to know if it is possible to simulate blockchain network on NS3 and if yes, can anyone share some links to tutorials that would be helpful for this. It would be helpful...

08 March 2020 7,384 1 View

Modeling of benzene recovery process by extractive distillation?

I am trying to model a Benzene recovery process by extractive distillation in Aspen plus. The calculation type chosen for the radfrac column is 'equilibrium' with the tray efficiency of 75%. i...

04 February 2020 9,279 3 View

Can i grow hacat and L929 cells with dmem high glucose + 4mM glutamine?

I am gonna grow hacat and L929 cells and can i grow these cell line with dmem high glucose+ 4mM L glutamine? Which is more preferable 2mM or 4mM glutamine? Thanks in advance.

09 March 2019 4,028 3 View

Can I use DMEM high glucose + 4M glutamine in culturing the L929 cell line??

I am going to grow L929 cells for scratch assay and i have been told that i need to grow them on DMEM low glucose with 2M glutamine and 10% fbs . can i grow it on high glucose with 4M glutamine...

27 February 2019 6,480 2 View

How to add randomness to a channel using Matlab simulations?

I have to simulate multipath fading channels and add randomness to it.

21 September 2018 6,377 2 View

Can someone give me suggestion over following choice experiment as to how I can place the variables in a right way?

Please suggest me how can I better place the variables in the following choice experiment. CE1. Please look at the following scenario and select the level of self-driving car you would consider...

03 April 2018 1,864 2 View

How to get rid of bubbles in plastic film?

Hi, What could be the reasons that a film prepared by casting a film forming solution on petri plate gets bubbles into it? How can it be prevented? Thanks, Ashish

08 February 2018 3,804 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View