Hello Reza

A PCR-based approach requires designing and ordering separate primers in addition to optimizing each reaction. This will take a lot of time regarding 50-100 SNPs. After spending these times, the fastest approach with clinical approval would be High Resolution Melt method. It is fast and reliable. Other PCR-based methods such as ARMS, may not be suitable for diagnostic purposes.

Hope it helped.

Hey there,

probably a custom made KIT for panel based sequencing is what you are looking for. Have a look at ArcherDX for example. But I think also other companies have offers like this.

Hope this helps a bit.

Greetings,

André

thank you Ali Javadmanesh . I followed your suggestion and found it fit to what I am looking for. Based on HRM approach, there are some commercial solutions to make an order for a customized kit, however the number of SNPs still matters! I know it would be possible using NGS and sequencing techniques, but I want to find the most cost-effective and precise method. Since it is intended for performing in Iran, I guess this should be considered as well! So, I would appreciate if you let me ask you more technical details by email.

Hi André Marquardt, I do appreciate your guidance.

Dear Reza,

SNPChip designed panel is the thing you want.

It is posible to order a panel with specific primers or use a panel set that may include your mentioned SNPs beside other SNPs.

So you dont want to consider microarray or NGS? then HRM genotyping using ABI quant-studio Realtime Machine might be your choice to multiplex upto 15 SNP in eah rxn using taqman. I imagine Ion torrent has specific panels for couple of hundreds SNP available for human.

https://www.gene-quantification.de/lifetech-quantstudio-brochure.pdf

below is an example of ion torrent mid throughput genotyping by sequencing.

Article Massively parallel sequencing of 231 autosomal SNPs with a c...