What is the oxygen diffusion constant in tissue culture media with 10 % serum at 37 °C? I could find value for water (2.86 × 10⁻⁵ cm²/s, https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204269#pone.0204269.ref032). But nothing for cell culture media...
Dear Václav Bočan
The oxygen diffusion coefficient of culture media will be less than that of water at 37°C because of the presence of solutes in cell culture media, such as salts, glucose, and serum.
Please refer to the article attached below.
https://pmc.ncbi.nlm.nih.gov/articles/PMC5699948/#:~:text=In%20the%20following%20sections%2C%20we,oxygen%20flux%20through%20the%20system.
Highlight:
The failures of the cell culture model with regard to oxygen diffusion can be best understood by examining Fick’s Law. Fick’s Law states that the rate of diffusion or flux (F) of a gas through a medium is directly proportional to the difference in concentration of the gas on either side of the medium, and inversely proportional to the thickness of the medium. The diffusion constant (D) corrects for the solubility of the gas in the medium.
F=D×Δ𝐶/Δ𝑥Fick’s First Law
In isotonic saline (which has a similar salt concentration to cell culture medium), D = 2.84 x10−5 cm2/s at 37ºC. The addition of 10% fetal bovine serum (FBS) makes the protein concentration 0.45 mg/dL, as the total protein concentration of FBS is 3.0–4.5 mg/dL. By extrapolating from data obtained by Goldstick et al., the value of D for a saline solution containing 0.45 mg/dL total protein is approximately 2.69 × 10−5 at 37ºC.
Regards,
Malcolm Nobre
- Badges
- Science topic