other than DNA gel extraction
What is the best method for DNA isolation from fish samples? Any experience and/or tips how to improve yield of genomic DNA from fish samples? What tissue is the best source? How much DNA is...
31 January 2023 5,759 1 View
The number of stable G-quadruplex prone motifs was strongly underestimated in the previous version of the human X-chromosome: Novel G-quadruplex prone sequences emerge in the complete as... Will...
07 October 2021 6,981 0 View
Hello, we are growing tomato in the greenhouse. Macrolophus pygmaeus is our main tool to control pests. We are at the moment for some un-known reason struggling to build up a strong popoulation of...
16 March 2020 7,986 6 View
Hello, we are facing to some problem with Macrolophus pygmaeus population in the greenhouse. We have introduce Macrolophus bug into the tomato greenhouse 5 months ago and reintroduce it about 4...
16 March 2020 7,216 3 View
I could find very old electron microscopy articles (like Gall 1966: Microtubule fine structure), but wanted to cite also some papers from last decade. But no one is citing the common fact that...
21 March 2019 3,120 3 View
I am interested in clay minerals formed in plutonic rocks (mainly granites) prior to obvious weatherig processes. If we look at wet analyses, there is mostly 1 wt. % "H2O+" (even in samples from...
01 December 2017 3,677 6 View
Hello, I need your opinon about pest which is causing damage on apple fruit. It is usually a hole with the cavity underneath. There are no excrements nor on fruit surface close the hole neither in...
15 September 2017 9,779 2 View
Hello everbody, I would like to find out what virus species attacked tomato. Please see pictures. Thank you for help.
09 February 2017 1,109 3 View
We will test this product within some trails in central Europe. I can´t find any materials in English. How to spray with this product?
17 May 2015 9,800 4 View
IndoorGML is new standard for indoor navigation network. I have building obejct in format obj, skp and I need create indoorGML. What application? Transform format? Thanks
04 February 2015 1,894 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
