I could find very old electron microscopy articles (like Gall 1966: Microtubule fine structure), but wanted to cite also some papers from last decade. But no one is citing the common fact that microtubules have diameter of ~25 nm...
Hey Vaclav,
Good point. I guess people base their dimension based on mainly following papers.
1. Amos, LA, Baker, TS ( 1979) The three‐dimensional structure of tubulin protofilaments. Nature279: 607– 612.
2. Beese, L, Stubbs, G, Cohen, C ( 1987) Microtubule structure at 18 Å resolution. J Mol Biol 194: 257– 264.
3. Hirose, K, Amos, WB, Lockhart, A, Cross, RA, Amos, LA ( 1997) Three‐dimensional cryoelectron microscopy of 16‐protofilament microtubules: structure, polarity and interaction with motor proteins. J Struct Biol 118: 140– 148.
4. Nogales, E, Whittaker, M, Milligan, RA, Downing, KH ( 1999) High resolution structure of the microtubule. Cell 96: 79– 88.
Cheers,
Mayank
Thanks a lot, Maynak.
Seems like citing older papers is the only availability.
Václav
You could look on AFM side:
Chapter 9: High-Resolution Imaging of Microtubules and Cytoskeleton Structures by Atomic Force Microscopy [Book]
Hamon, Loic; Curmi, Patrick A.; Pastré, David
In Microtubules, in vitro, Methods in Cell Biology 2010 95:157-174
other than DNA gel extraction
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