In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over cell and tissue as a important component of lysis buffer.
For your convenient answering, I am working on H9C2 cell lines. And, now I need to prepare sample by using lysis buffer to perform western blot.
So, I want to know that, which concentration and storage condition should I follow and why?