Hello Yashila Dewan

Dexamethasone is soluble in DMSO (25mg/ml).

The molecular weight of Dexamethasone is 392.464g/mol.

Then follow the equation given below.

392.464g---- 1Mol---- 1L

3.924g---- 0.01M---- 1000 ml

3924mg---- 0.01M----- 1000ml

3.924mg----- 0.01M-----1ml

So, weigh 3.924mg of Dexamethasone and dissolve in 1ml of DMSO to obtain 0.01M dexamethasone stock solution in DMSO.

When you prepare working solution of Dexamethasone from the stock solution which contains 100% DMSO, the final DMSO concentration to be used for dendritic cells in culture should be less than or equal to 0.1%. Use of 0.1% DMSO is considered to be safe for almost all cells without any severe toxicity to the cells.

So, prepare working solution of Dexamethasone by diluting the stock solution with culture media in such a way that the DMSO concentration is well within 0.1% when finally added to dendritic cells in culture.

Best.