Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

How To Find Electrical Nanodipole and Magnetic Loop Dimensions At Optical Wavelength?

I would be grateful if you could suggest a paper or book which provides an equation to determine the dimensions (length) of an electrical nanodipole and magnetic loop antenna at desired optical...

17 October 2020 2,421 4 View

What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

Can anyone advise me in selecting a good pair of universal primer/primers for identification of lactic acid bacteria (probiotics)?

when I blast the existed primers specially 16s rDNAs , I cant get good results.

21 September 2020 1,584 4 View

Could anyone help me searching for protocols in fiber analyses ?

Hello, I am looking for protcoles to evaluate the following components: TDF : total dietary fiber, SDF : soluble dietary fiber, IDF : insoluble dietary fiber et TDN : total digestive nutrients.

06 September 2020 371 2 View

Any journal which has no publication fess for pops removal or water treatment through adsorption?

I am looking for a reputable journal for publication of research article of MS thesis. Kindly suggest me some journals related to water treatment through adsorption which is free of cost.

13 July 2020 8,259 6 View

How much water we should add to paint if we use a based water binder (resin) or there is no need for adding water with such binder?

I want to know if the water included in resin is enough as a solvent or that water is not counted and I should add water as solvent to paint?

21 June 2020 322 6 View

In your opinion, do I choose a certain disorder to study its relationship with culture, or better not to define the disorder?

Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)

26 April 2020 3,003 36 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View