What is the optimal method for using flow cytometry with adiopcytes to measure the expression of CD markers?

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What are gaps in diabetic wound healing techniques or dressings formation research?

Wound Healing research gaps using some polymers

28 July 2024 5,178 0 View

When using provean bioinformatic tool, what is meant by the amino acid variation?

After entering the protein sequence in the provean tool, it is followed by amino acid variation in order to run it and get the score. which are these variations?

11 June 2024 5,467 1 View

Je veux des articles et des ouvrages en rapport avec mon sujet de recherche?

voilà les mots clés: la communication ,sage femme; technolecte

27 May 2024 1,740 1 View

Is there anyone can help me with finding some samples of source codes or designed projects for OMnet++, SUMO, VANET Routing Protocol ?

I am trying to compare the performance of routing protocol like AODV, GPSR , DSDV , DYMO in VANET using OMNET++

19 May 2024 8,866 2 View

About subtypes of Dyslexia : Single article containing: "Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...?

I am unable to find a source containing all subtypes of dyslexia ("Double Deficit Dyslexia," "Surface Dyslexia", "Rapid Naming Deficit"...) in a single article to use as a reference for my...

27 March 2024 8,454 2 View

How to extract data from figures with high accuracy?

Such as studies that represent results (mean, standard deviation) with graphs rather than numerical data

08 March 2024 3,555 1 View

Apoptosis assay using flow cytometry, can I use annexin FITC in GFP fluorescent cells?

I have transfected cells with GFP, which emit in close range to FITC and used apoptosis kit that have annexin conjugated with FITC to measure apoptosis and PI to measure death. I am gating for GFP...

07 March 2024 9,673 5 View

How to recover single strand dna after AMpure XP Beads purification ?

Hello! I have a problem with my purification step. I have a single strand dna (after a digestion using Lambda Exonuclease) that I need to purify before injecting it as a dna donnor in C.elegans...

06 March 2024 5,529 0 View

Do you suggest that I order my kit from BT Lab?

I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.

27 February 2024 6,940 1 View

If i mix magnetite and maghemite nanoparticle using silica as matrix does it consider as nanocomposite?

to find a research gap i want to mix mnagnetite and maghemite nanoparticle and mix them with silica as matrix does it considered as nanocomposite?

23 February 2024 2,205 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

I came across oscillations in a pressure profile for a pipeline flow along the axis of a cylinder, how do I prove that these are not numerical err?

In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?

13 July 2024 6,296 5 View