Any advice on the optimal concentrations of PHA to use in Jurkat cell culture which is being stimulated for 48 hours for the analysis of early activation marker CD69 using flow cytometry. Any recommendations would be greatly appreciated.
Piero Pileri
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Jurkat cells can be stimulated with 1 µg/ml PHA plus 10 ng/ml PMA or 10 µg/ml anti-CD3ε (mAb) (OKT3) plus 10 µg/ml anti-CD28 mAb
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Piero Pileri
Jurkat cells can be stimulated with 1 µg/ml PHA plus 10 ng/ml PMA or 10 µg/ml anti-CD3ε (mAb) (OKT3) plus 10 µg/ml anti-CD28 mAb
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Piero Pileri
I post the link of a more exhaustive discussion on this argument
https://www.researchgate.net/post/Unspecific_stimulation_with_PMA_ionomycin_vs_PHA_for_intracellular_cytokine_staining_of_T_cells/1
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