Neha Chaudhary

3 Questions 7 Answers 0 Followers

Questions related from Neha Chaudhary

Can anyone please recommend a protocol for Raji B-cells stimulation with PMA and ionomycin?

I am looking for a protocol for the stimulation of Raji B-cells with PMA and Ionomycin. I want to observe CD69 early activation marker using flow cytometry.

09 September 2018 5,960 0 View

How can we activate jurkat cells using PHA and assess CD69 marker?

I am unable to find a good literature explaining the protocol for activating Jurkat cells using PHA and assessing the activation through CD69 marker.

09 September 2016 4,343 4 View

What is the optimal concentration of PHA to activate Jurkat cell culture and concentration of anti-human CD69 to assess CD69 marker ?

Any advice on the optimal concentrations of PHA to use in Jurkat cell culture which is being stimulated for 48 hours for the analysis of early activation marker CD69 using flow cytometry. Any...

09 September 2016 7,030 4 View