I am using the Covaris S220 with a 1ml Glass Tube Cat#520056 to shear crosslinked yeast chromatin. I have a lot to optimize going into this protocol with a new strain but found only one source that recommended a range of 0.5-1.5e7 cells/ml for mammalian cells.
Is this a good place to start? Do you have a better recommendation for yeast?
PS:
Currently my setting are as follows if that helps.
1ml, PIP:240W, DF:20%, CPB:200, 4C