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Questions related from Jacob Bassett
I hope others with real world experience can share the pros and cons of their preferred protease. My criteria... -Speed of lysis -Robustness(pH, temperature, and salinity compatibility) -Storage...
01 September 2021 1,642 2 View
I am using the Covaris S220 with a 1ml Glass Tube Cat#520056 to shear crosslinked yeast chromatin. I have a lot to optimize going into this protocol with a new strain but found only one source...
29 February 2020 7,200 1 View
I did a trial run of a his-purification and found that my protein would be a little dilute for my purposes. Any thoughts on the best way to concentrate a denatured protein? This is my buffer and...
17 October 2017 5,490 7 View
Any good ideas as to what is causing these streak would be helpful. Thanks Details: Second step of a taptag purification Bis-Tris Precast Polyacrylamide gels Ran at 165 volts 1ul sample/20ul...
09 January 2015 2,571 7 View