Jacob Bassett

4 Questions 5 Answers 0 Followers

Questions related from Jacob Bassett

Best protease to elute a native protein?

I hope others with real world experience can share the pros and cons of their preferred protease. My criteria... -Speed of lysis -Robustness(pH, temperature, and salinity compatibility) -Storage...

01 September 2021 1,646 2 View

What is the optimal concentration of chromatin for shearing?

I am using the Covaris S220 with a 1ml Glass Tube Cat#520056 to shear crosslinked yeast chromatin. I have a lot to optimize going into this protocol with a new strain but found only one source...

29 February 2020 7,207 1 View

How to concentrate a denatured his-purified protein?

I did a trial run of a his-purification and found that my protein would be a little dilute for my purposes. Any thoughts on the best way to concentrate a denatured protein? This is my buffer and...

17 October 2017 5,498 7 View

What are these streaks on silver stained polyacrylamide gels?

Any good ideas as to what is causing these streak would be helpful. Thanks Details: Second step of a taptag purification Bis-Tris Precast Polyacrylamide gels Ran at 165 volts 1ul sample/20ul...

09 January 2015 2,576 7 View